Isoform-specific and signaling-dependent propagation of acute myeloid leukemia by Wilms tumor 1.
Potluri S, Assi SA, Chin PS, Coleman DJL, Pickin A, Moriya S, Seki N, Heidenreich O, Cockerill PN, Bonifer C
Cell Rep. 2021 Apr 20;35(3):109010. doi: 10.1016/j.celrep.2021.109010.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 217636 | pTRIPZ-shWT1_A | Dox inducible shRNA targeting WT-1 |
| 217637 | pTRIPZ-shWT1_B | Dox inducible shRNA targeting WT-1 |
| 217638 | pTRIPZ-shNTC | Dox inducible shRNA non targeting control shRNA |
| 217639 | pENTR-GFP-WT1 +KTS N terminal HA | Entry vector with WT1 +KTS mutant with N terminal HA for gateway cloning |
| 217640 | pENTR-GFP-WT1 +KTS C terminal HA | Entry vector with WT1 +KTS mutant with C terminal HA for gateway cloning |
| 217641 | pENTR-GFP-WT1 -KTS N terminal HA | Entry vector with WT1 -KTS mutant with N terminal HA for gateway cloning |
| 217642 | pENTR-GFP-WT1 -KTS C terminal HA | Entry vector with WT1 -KTS mutant with C terminal HA for gateway cloning |
| 217643 | pCW57.1 WT1 +1 ATG | Dox inducible WT1 cDNA - as transcribed from +1ATG (long isoform) |
| 217644 | pCW57.1 WT1 +378 ATG | Dox inducible WT1 cDNA - as transcribed from +378ATG (short isoform) |
| 217645 | pCW57.1 Ex8 mut WT1 | Dox inducible WT1 cDNA with exon 8 mutation |
| 217646 | pCW57.1 Ex9 mut WT1 | Dox inducible WT1 cDNA with exon 9 mutation |