Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s
Lin GM, Voigt CA
Nature Catalysis. 6, 1016–1029 (2023). https://doi.org/10.1038/s41929-023-01049-5
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197112 | E. coli MEV15 | E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster |
| 197113 | E. coli MEV20 | E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster |
| 208257 | pLRD01 | Production of terpentetriene in E. coli MEV20 |
| 208258 | pLRD04 | Production of ent-kaurene in E. coli MEV20 |
| 208259 | pLRD05 | Production of isopimara-8,15-diene in E. coli MEV20 |
| 208260 | pLRD07 | Production of (16R)-ent-kauran-16-ol in E. coli MEV20 |
| 208261 | pLRD09 | Production of biformene in E. coli MEV20 |
| 208262 | pLRD10 | Production of labda-7,13(16),14-triene in E. coli MEV20 |
| 208263 | pLRD11 | Production of sclareol in E. coli MEV20 |
| 208264 | pLRD12 | Production of miltiradiene in E. coli MEV20 |
| 208265 | pLRD13 | Production of ent-cassa-12,15-diene in E. coli MEV20 |
| 208266 | pLRD14 | Production of syn-pimara-7,15-diene in E. coli MEV20 |
| 208267 | pLRD15 | Production of ent-sandaracopimaradiene in E. coli MEV20 |
| 208268 | pLRD16 | Production of syn-stemar-13-ene in E. coli MEV20 |
| 208269 | pLRD17 | Production of syn-stemod-13(17)-ene in E. coli MEV20 |
| 208270 | pLRD18 | Production of ent-isokaurene in E. coli MEV20 |
| 208271 | pLRD20 | Production of 13R-manoyl oxide in E. coli MEV20 |
| 208272 | pLRD25 | Production of cis-abienol in E. coli MEV20 |
| 208273 | pLRD26 | Production of levopimaradiene in E. coli MEV20 |
| 212834 | pCYP01 | Expresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzyme |
| 212835 | pCYP02 | Expresses the Sinorhizobium fredii LRD-modifying cytochrome P450 enzyme |
| 212836 | pCYP03 | Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme |
| 212837 | pCYP04 | Expresses the Rhizobium grahamii LRD-modifying cytochrome P450 enzyme |
| 212838 | pCYP05 | Expresses the Burkholderia multivorans LRD-modifying cytochrome P450 enzyme |
| 212839 | pCYP06 | Expresses the Rhizobium acidisoli LRD-modifying cytochrome P450 enzyme |
| 212840 | pCYP07 | Expresses the Bradyrhizobium sp. Rc3b LRD-modifying cytochrome P450 enzyme |
| 212841 | pCYP08 | Expresses the Sinorhizobium fredii LRD-modifying cytochrome P450 enzyme |
| 212842 | pCYP09 | Expresses the Mesorhizobium sp. LSJC277A00 LRD-modifying cytochrome P450 enzyme |
| 212843 | pCYP10 | Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme |
| 212844 | pCYP11 | Expresses the Rhizobium mesoamericanum LRD-modifying cytochrome P450 enzyme |
| 212845 | pCYP12 | Expresses the Bradyrhizobium sp. Rc3b LRD-modifying cytochrome P450 enzyme |
| 212846 | pCYP13 | Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme |
| 212847 | pCYP14 | Expresses the Sinorhizobium fredii LRD-modifying cytochrome P450 enzyme |
| 212848 | pCYP15 | Expresses the Micromonospora cremea LRD-modifying cytochrome P450 enzyme |
| 212849 | pCYP16 | Expresses the Actinomadura pelletieri LRD-modifying cytochrome P450 enzyme |
| 212850 | pCYP17 | Expresses the Actinomadura sp. KC06 LRD-modifying cytochrome P450 enzyme |
| 212851 | pCYP18 | Expresses the Nonomuraea mesophila LRD-modifying cytochrome P450 enzyme |
| 212852 | pCYP19 | Expresses the Sphaerisporangium album LRD-modifying cytochrome P450 enzyme |
| 212853 | pCYP20 | Expresses the Thermomonospora curvata LRD-modifying cytochrome P450 enzyme |
| 212854 | pCYP21 | Expresses the Nonomuraea jiangxiensis LRD-modifying cytochrome P450 enzyme |
| 212855 | pCYP22 | Expresses the Scytonema sp. HK-05 LRD-modifying cytochrome P450 enzyme |
| 212856 | pCYP23 | Expresses the Streptomyces atratus LRD-modifying cytochrome P450 enzyme |
| 212857 | pCYP24 | Expresses the Streptomyces cyslabdanicus LRD-modifying cytochrome P450 enzyme |
| 212858 | pCYP25 | Expresses the Streptomyces sp. HB202 LRD-modifying cytochrome P450 enzyme |
| 212859 | pCYP26 | Expresses the Streptomyces sp. SceaMP-e96 LRD-modifying cytochrome P450 enzyme |
| 212860 | pCYP27 | Expresses the Kitasatospora sp. CB02891 LRD-modifying cytochrome P450 enzyme |
| 212861 | pCYP28 | Expresses the Micromonospora humi LRD-modifying cytochrome P450 enzyme |
| 212862 | pCYP29 | Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme |
| 212863 | pCYP30 | Expresses the Streptomyces albireticuli LRD-modifying cytochrome P450 enzyme |
| 212864 | pCYP31 | Expresses the Saccharomonospora piscinae LRD-modifying cytochrome P450 enzyme |
| 212865 | pCYP32 | Expresses the Prauserella muralis LRD-modifying cytochrome P450 enzyme |
| 212866 | pCYP33 | Expresses the Streptomyces platensis LRD-modifying cytochrome P450 enzyme |
| 212867 | pCYP34 | Expresses the Streptomyces platensis LRD-modifying cytochrome P450 enzyme |
| 212868 | pCYP35 | Expresses the Saccharopolyspora antimicrobica LRD-modifying cytochrome P450 enzyme |
| 212869 | pCYP36 | Expresses the Saccharopolyspora elongata LRD-modifying cytochrome P450 enzyme |
| 212870 | pCYP37 | Expresses the Nocardia terpenica LRD-modifying cytochrome P450 enzyme |
| 212871 | pCYP38 | Expresses the Xanthomonas translucens pv. phlei. LRD-modifying cytochrome P450 enzyme |
| 212872 | pCYP39 | Expresses the Saccharothrix texasensis LRD-modifying cytochrome P450 enzyme |
| 212873 | pCYP40 | Expresses the Micromonospora mirobrigensis LRD-modifying cytochrome P450 enzyme |
| 212874 | pCYP41 | Expresses the Saccharothrix texasensis LRD-modifying cytochrome P450 enzyme |
| 212875 | pCYP42 | Expresses the Micromonospora humi LRD-modifying cytochrome P450 enzyme |
| 212876 | pCYP43 | Expresses the Pseudonocardia sp. Ae717_Ps2 LRD-modifying cytochrome P450 enzyme |
| 212877 | pCYP44 | Expresses the Saccharopolyspora shandongensis LRD-modifying cytochrome P450 enzyme |
| 212878 | pCYP45 | Expresses the Streptomyces sp. BK447 LRD-modifying cytochrome P450 enzyme |
| 212879 | pCYP46 | Expresses the Streptomyces cyslabdanicus LRD-modifying cytochrome P450 enzyme |
| 212880 | pCYP47 | Expresses the Streptomyces olivaceus LRD-modifying cytochrome P450 enzyme |
| 212881 | pCYP48 | Expresses the Streptomyces sp. Tu6071 LRD-modifying cytochrome P450 enzyme |
| 212882 | pCYP49 | Expresses the Streptomyces sp. AVP053U2 LRD-modifying cytochrome P450 enzyme |
| 212883 | pCYP50 | Expresses the Lechevalieria deserti LRD-modifying cytochrome P450 enzyme |
| 212884 | pCYP51 | Expresses the Kitasatospora sp. CB02891 LRD-modifying cytochrome P450 enzyme |
| 212885 | pCYP52 | Expresses the Herbihabitans rhizosphaerae LRD-modifying cytochrome P450 enzyme |
| 212886 | pCYP53 | Expresses the Streptomyces argenteolus LRD-modifying cytochrome P450 enzyme |
| 212887 | pCYP54 | Expresses the Streptomyces argenteolus LRD-modifying cytochrome P450 enzyme |
| 212888 | pCYP55 | Expresses the Salinispora arenicola LRD-modifying cytochrome P450 enzyme |
| 212889 | pCYP56 | Expresses the Rhizobium meliloti LRD-modifying cytochrome P450 enzyme |
| 212890 | pCYP57 | Expresses the Mesorhizobium japonicum LRD-modifying cytochrome P450 enzyme |
| 212891 | pCYP58 | Expresses the Streptomyces griseolus LRD-modifying cytochrome P450 enzyme |
| 212892 | pCYP59 | Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme |
| 212893 | pCYP60 | Expresses the Kitasatospora griseola LRD-modifying cytochrome P450 enzyme |
| 212894 | pCYP61 | Expresses the Kitasatospora griseola LRD-modifying cytochrome P450 enzyme |
| 212895 | pCYP62 | Expresses the Streptomyces sp. KO-3988 LRD-modifying cytochrome P450 enzyme |
| 212896 | pCYP63 | Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme |
| 212897 | pCYP64 | Expresses the Priestia megaterium LRD-modifying cytochrome P450 enzyme |