Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: application for betanin biosynthesis.
Jiang W, Wang S, Avila P, Jorgensen TS, Yang Z, Borodina I
Metab Eng. 2024 Sep 9:S1096-7176(24)00110-1. doi: 10.1016/j.ymben.2024.09.003.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
These materials are included in the EXPRESSYALI kit.
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
212676 | pCfB13089 | TEF promoter |
212677 | pCfB12706 | EXP promoter |
212678 | pCfB12708 | GPD promoter |
212679 | pCfB13090 | Lip2 terminator |
212680 | pCfB12710 | Syn8 terminator |
212681 | PCfB12709 | SynGuo terminator |
212682 | pCfB12711 | integration vector for single cassette with integration site E2 |
212683 | pCfB12712 | integration vector for single cassette with integration site E2 |
212684 | pCfB12713 | integration vector for single cassette with integration site E2 |
212685 | pCfB13014 | integration vector for single cassette with integration site E3 |
212686 | pCfB13015 | integration vector for single cassette with integration site E3 |
212687 | pCfB13016 | integration vector for single cassette with integration site E3 |
212688 | pCfB12714 | integration vector for three gene cassette with integration site E2 |
212689 | pCfB12715 | integration vector for three gene cassette with integration site E3 |
212690 | pCfB12716 | integration vector for three gene cassette with integration site E4 |
212691 | pCfB12717 | integration vector for three gene cassette with integration site C3 |
212692 | pCfB12718 | integration vector for three gene cassette with integration site F3 |
212693 | pCfB13017 | integration vector for three gene cassette with integration site of the gene 4HPPD locus |
212694 | pCfB10769 | gRNA targeting to the intergradtion site E2 |
212695 | pCfB11787 | gRNA targeting to the intergradtion site E2 |
212696 | pCfB6637 | gRNA targeting to the intergradtion site E3 |
212697 | pCfB8860 | gRNA targeting to the intergradtion site E3 |
212698 | pCfB6638 | gRNA targeting to the intergradtion site E4 |
212699 | pCfB11785 | gRNA targeting to the intergradtion site E4 |
212700 | pCfB6630 | gRNA targeting to the intergradtion site C3 |
212701 | pCfB8856 | gRNA targeting to the intergradtion site C3 |
212702 | pCfB12719 | gRNA targeting to the intergradtion site F3 |
212703 | pCfB8866 | gRNA targeting to the intergradtion site F3 |
212704 | pCfB12720 | gRNA targeting to the gene 4HPPD locus |