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Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: application for betanin biosynthesis.

Jiang W, Wang S, Avila P, Jorgensen TS, Yang Z, Borodina I
Metab Eng. 2024 Sep 9:S1096-7176(24)00110-1. doi: 10.1016/j.ymben.2024.09.003. (Link opens in a new window) PubMed (Link opens in a new window) Article


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Plasmids from Article

ID Plasmid Purpose
212676pCfB13089TEF promoter
212677pCfB12706EXP promoter
212678pCfB12708GPD promoter
212679pCfB13090Lip2 terminator
212680pCfB12710Syn8 terminator
212681PCfB12709SynGuo terminator
212682pCfB12711integration vector for single cassette with integration site E2
212683pCfB12712integration vector for single cassette with integration site E2
212684pCfB12713integration vector for single cassette with integration site E2
212685pCfB13014integration vector for single cassette with integration site E3
212686pCfB13015integration vector for single cassette with integration site E3
212687pCfB13016integration vector for single cassette with integration site E3
212688pCfB12714integration vector for three gene cassette with integration site E2
212689pCfB12715integration vector for three gene cassette with integration site E3
212690pCfB12716integration vector for three gene cassette with integration site E4
212691pCfB12717integration vector for three gene cassette with integration site C3
212692pCfB12718integration vector for three gene cassette with integration site F3
212693pCfB13017integration vector for three gene cassette with integration site of the gene 4HPPD locus
212694pCfB10769gRNA targeting to the intergradtion site E2
212695pCfB11787gRNA targeting to the intergradtion site E2
212696pCfB6637gRNA targeting to the intergradtion site E3
212697pCfB8860gRNA targeting to the intergradtion site E3
212698pCfB6638gRNA targeting to the intergradtion site E4
212699pCfB11785gRNA targeting to the intergradtion site E4
212700pCfB6630gRNA targeting to the intergradtion site C3
212701pCfB8856gRNA targeting to the intergradtion site C3
212702pCfB12719gRNA targeting to the intergradtion site F3
212703pCfB8866gRNA targeting to the intergradtion site F3
212704pCfB12720gRNA targeting to the gene 4HPPD locus

Antibodies from Article