Highly efficient cellular expression of circular mRNA enables prolonged protein expression.

Unti MJ, Jaffrey SR
Cell Chem Biol. 2023 Oct 13:S2451-9456(23)00334-3. doi: 10.1016/j.chembiol.2023.09.015. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
212611	pcDNA3.1+-Tornado-nLuc	For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.
212612	pcDNA3.1+-Tornado-split-nLuc	For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.
212707	pcDNA3.1+-mutTornado-split-nLuc	Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.