Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi.
Edenhofer FC, Termeg A, Ohnuki M, Jocher J, Kliesmete Z, Briem E, Hellmann I, Enard W
iScience. 2024 May 23;27(6):110090. doi: 10.1016/j.isci.2024.110090. eCollection 2024 Jun 21.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212829 | pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry | Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome. |
| 212830 | pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry | Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome. |
| 212831 | pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry | Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome. |