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Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

Edenhofer F. C., Térmeg A., Ohnuki M., Jocher J., Kliesmete Z., Briem E., Hellmann I., Enard W.
iScience, 21 June 2024 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
212829pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherryDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.
212830pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherryDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.
212831pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherryDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

Antibodies from Article