Programmable RNA-guided DNA endonucleases are widespread in eukaryotes and their viruses.
Jiang K, Lim J, Sgrizzi S, Trinh M, Kayabolen A, Yutin N, Bao W, Kato K, Koonin EV, Gootenberg JS, Abudayyeh OO
Sci Adv. 2023 Sep 29;9(39):eadk0171. doi: 10.1126/sciadv.adk0171. Epub 2023 Sep 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212315 | pDY1935 | Express ApmFnuc protein with sumotag |
| 212316 | pKJ226 | Express DpFnuc with pureexpress |
| 212318 | pKJ217 | Express MmFnuc with pureexpress |
| 212319 | pKJ221 | Express MmFnuc guide with pureexpress |
| 212320 | pKJ237 | Express KnFnuc |
| 212321 | pKJ238 | Express KnFnuc guide with pureexpress |
| 217492 | pKJ234 | Mammlian expression of DpFnuc for genome editing |
| 217493 | pKJ235 | Mammlian expression of MmFnuc for genome editing |
| 217494 | pKJ248 | Mammlian expression of DpFnuc guide RNA |
| 217495 | pKJ318 | Mammlian expression of MmFnuc guide RNA |
| 217496 | pKJ224 | Bacterial expression of BaFnuc for IVTT |
| 217497 | pKJ223 | Bacterial expression of BaFnuc guide RNA |