Live-cell transcriptomics with engineered virus-like particles
Najia MA, Borrajo J, Le A, Tsai F, Huang JY, Griffith LG, Daley GQ, Blainey PC
bioRxiv 2024.10.01.616098
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 212626 | CROPseq-Puro-4xMS2 | MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles |
| 212627 | CROPseq-Puro-2xMS2 | MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles |
| 212628 | CROPseq-Puro-12xMS2 | MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles |
| 212629 | CROPseq-Puro-24xMS2 | MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles |
| 212630 | pGag | Expression vector for MLV Gag |
| 212631 | pGag-mCherry | Expression vector for an MLV Gag-mCherry fusion protein |
| 212632 | pGag∆MA | Expression vector for MLV Gag with truncated MA domain |
| 212633 | pLV_CAG_Gag∆MA | Lentivirus vector to express MLV Gag with truncated MA domain |
| 212634 | pLV_CAG_Gag | Lentivirus vector to express MLV Gag |
| 212637 | pLV_CAG_Gag-MCP | Lentivirus vector to express an MLV Gag-MCP fusion protein |
| 212638 | pLV_EF1a_Gag | Lentivirus vector to express MLV Gag |
| 212640 | pLV_EF1a_mNeon | Lentivirus vector to express mNeon |
| 212641 | pFLAG-VSVG | Expression vector for FLAG-VSVG |
| 212642 | pLV_CMV_FLAG-VSVG | Lentivirus vector to express FLAG-VSVG |
| 212643 | pLV_CMV_His-VSVG | Lentivirus vector to express His-VSVG |
| 212644 | pLV_CMV_cMyc-VSVG | Lentivirus vector to express cMyc-VSVG |
| 212645 | pLV_CMV_HA-VSVG | Lentivirus vector to express HA-VSVG |
| 212646 | pPB_EF1a_FLAG-VSVG | piggyBac vector to express FLAG-VSVG |
| 212647 | pPB_EF1a_HA-VSVG | piggyBac vector to express HA-VSVG |
| 212648 | pPB_EF1a_tNGFR_ZeoR | piggyBac vector to express tNGFR with zeocin resistance |
| 212649 | pPB_EF1a_FLAG-tNGFR_ZeoR | piggyBac vector to express FLAG-tNGFR with zeocin resistance |
| 212650 | pPB_EF1a_HA-tNGFR_ZeoR | piggyBac vector to express HA-tNGFR with zeocin resistance |
| 212651 | pPB_EF1a_FLAG-tNGFR_PuroR | piggyBac vector to express FLAG-tNGFR with puromycin resistance |
| 212652 | pPB_EF1a_HA-tNGFR_PuroR | piggyBac vector to express HA-tNGFR with puromycin resistance |
| 212653 | pPB_EF1a_mNeon | piggyBac vector to express mNeon |
| 212654 | pPB_EF1a_Gag∆MA | piggyBac vector to express MLV Gag with truncated MA domain |
| 212656 | pPB_EF1a_coGag | piggyBac vector to express codon optimized MLV Gag |
| 212657 | pPB_EF1a_Gag-EIF4E | piggyBac vector to express an MLV Gag-EIF4E fusion protein |
| 212658 | pPB_EF1a_Gag-EIF4E-TM | piggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E) |