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Live-cell transcriptomics with engineered virus-like particles

Najia, M. A., Borrajo, J., Le, A., Tsai, F., Huang, J. Y., Griffith, L. G., Daley, G. Q., & Blainey, P. C.
bioRxiv. (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
212626CROPseq-Puro-4xMS2MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
212627CROPseq-Puro-2xMS2MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
212628CROPseq-Puro-12xMS2MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles
212631pGag-mCherryExpression vector for an MLV Gag-mCherry fusion protein
212637pLV_CAG_Gag-MCPLentivirus vector to express an MLV Gag-MCP fusion protein
212638pLV_EF1a_GagLentivirus vector to express MLV Gag
212640pLV_EF1a_mNeonLentivirus vector to express mNeon
212641pFLAG-VSVGExpression vector for FLAG-VSVG
212642pLV_CMV_FLAG-VSVGLentivirus vector to express FLAG-VSVG
212643pLV_CMV_His-VSVGLentivirus vector to express His-VSVG
212644pLV_CMV_cMyc-VSVGLentivirus vector to express cMyc-VSVG
212645pLV_CMV_HA-VSVGLentivirus vector to express HA-VSVG
212646pPB_EF1a_FLAG-VSVGpiggyBac vector to express FLAG-VSVG
212647pPB_EF1a_HA-VSVGpiggyBac vector to express HA-VSVG
212648pPB_EF1a_tNGFR_ZeoRpiggyBac vector to express tNGFR with zeocin resistance
212649pPB_EF1a_FLAG-tNGFR_ZeoRpiggyBac vector to express FLAG-tNGFR with zeocin resistance
212650pPB_EF1a_HA-tNGFR_ZeoRpiggyBac vector to express HA-tNGFR with zeocin resistance
212651pPB_EF1a_FLAG-tNGFR_PuroRpiggyBac vector to express FLAG-tNGFR with puromycin resistance
212653pPB_EF1a_mNeonpiggyBac vector to express mNeon
212654pPB_EF1a_Gag∆MApiggyBac vector to express MLV Gag with truncated MA domain
212656pPB_EF1a_coGagpiggyBac vector to express codon optimized MLV Gag
212657pPB_EF1a_Gag-EIF4EpiggyBac vector to express an MLV Gag-EIF4E fusion protein
212658pPB_EF1a_Gag-EIF4E-TMpiggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)

Antibodies from Article