Kang Zhou Lab: Harnessing xylitol utilization in Escherichia coli for antibiotic-free plasmid and recombinant protein production.

Unpublished

Plasmids from Article

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ID	Plasmid	Purpose
212155	P48	Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.
212153	PHB1	For PHB production in E. coli (PT7-pMB1).
212152	pCT02	Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
212150	pCT03	Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.
212149	pGU08	Plasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.
212148	pGU06	Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.
212146	PgyrA_gfp	Plasmid expressing green fluorescent protein under constitutive promoter PgyrA control.
212145	INT_SS9sp	Effector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
212144	INT_GFPsp	Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
212143	INT_GFPsp_CamRcargo	Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.