StayGold variants for molecular fusion and membrane-targeting applications.

Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A
Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
212017	pRSETB/mStayGold	mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.
212018	pRSETB/mStayGold(c4)	For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.
212019	pcDNA3/F-tractin=mStayGold	For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).
212020	pcDNA3/mStayGold(c4)=UtrCH	For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).
212022	pcDNA3/td5StayGold(c4)=GianCreg	For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].