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Rapid and Quantitative Functional Interrogation of Human Enhancer Variant Activity in Live Mice

Hollingsworth EW, Liu TA, Jacinto SH, Chen CX, Alcantara JA, Kvon EZ
bioRxiv 2023.12.10.570890 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
211940PCR4-Hsp68::mCherry-H11dual-enSERT-1 mCherry vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)
211941PCR4-Hsp68::eGFP-H11dual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)
211942PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)
216394PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct (human beta-globin promoter) into the H11 locus (separated by a synthetic insulator)

Antibodies from Article