Rapid depletion of target proteins in plants by an inducible protein degradation system.
Huang L, Rojas-Pierce M
Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211836 | pDGB3_alpha1-AtUBQ10:GFP-T35S | Plant transient expression cassette for GFP (control) under UBQ10 promoter. |
| 211837 | pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S | Plant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter. |
| 211839 | pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S | Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter. |
| 211840 | pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos | Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter. |
| 211841 | pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos | Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal. |
| 211842 | pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos | Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase |
| 211843 | pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos | Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter. |
| 211844 | pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos | Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal. |
| 211845 | pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos | Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive. |
| 211847 | pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter | Plant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degron |
| 211848 | pUPD2-GFP control (B3-B5) | GFP CDS in domestication vector |
| 211849 | pUPD2-N-L-mCherry2 (B5) | CDS for mCherry in domestication vector |
| 211851 | pUPD2-HR1b-C (B3-B4) | CDS for HR1b degron sequence in domestication vector |
| 211852 | pUPD2-N-L-HR1b (B4-B5) | CDS for HR1b degron sequence in domestication vector |
| 211853 | pUPD2-HR1b-GFP-L (B3-B4) | E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid |
| 211855 | pUPD2-HR1bR181A R185A-C (B3-B4) | CDS for mutant HR1b degron in domestication vector |
| 211856 | pUPD2-N-L-HR1bR181A R185A (B4-B5) | CDS for mutant HR1b degron in domestication vector |
| 211857 | pUPD2-HR1bR181A R185A-GFP-L (B3-B4) | Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid |
| 211859 | pUPD2-LRR-NEL (B4-B5) | CDS for E3-DART ligase in domestication vector |
| 211860 | pUPD2-HA-LRR-NEL-C (B3-B4) | CDS for E3-DART ligase sequence in domestication vector |
| 211861 | pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4) | Nuclear-localized E3-DART ligase in domestication vector. |
| 211862 | pUPD2-HA-LRR-NELC492A-C (B3-B4) | Mutant E3-DART ligase in domestication vector |
| 211863 | pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4) | Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector. |