Coupling during collective cell migration is controlled by a vinculin mechanochemical switch.

Shoyer TC, Gates EM, Cabe JI, Urs AN, Conway DE, Hoffman BD
Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 10.1073/pnas.2316456120. Epub 2023 Dec 6. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
211835	pRRL-VinculinTS-S1033A	Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
211893	pRRL-VinculinTS-S1033D	Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
211894	pRRL-VinculinCS-S1033A	Vinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
211895	pRRL-VinculinCS-S1033D	Vinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
211896	pRRL-VinculinVenus-S1033A	Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.
211897	pRRL-VinculinVenus-S1033D	Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.