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CO(2)-based production of phytase from highly stable expression plasmids in Cupriavidus necator H16.

Arhar S, Rauter T, Stolterfoht-Stock H, Lambauer V, Kratzer R, Winkler M, Karava M, Kourist R, Emmerstorfer-Augustin A
Microb Cell Fact. 2024 Jan 3;23(1):9. doi: 10.1186/s12934-023-02280-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
211826pKERepMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.
211827pKERepParMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.
211828pKESaLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.
211829pKESaParLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

Antibodies from Article