A modular dCas9-based recruitment platform for combinatorial epigenome editing.
Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li JK, Lister R
Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211766 | LLP773_pLenti-6xHRBKET-sgRNA | Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection |
| 211767 | LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP) | dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection |
| 211768 | LLP775_αGCN4-p65HSF1-CO | SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection |
| 211769 | LLP776_SpyCatcher-p65HSF1-CO | SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection |
| 211770 | LLP777_SnoopCatcher-p65HSF1-CO | SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection |
| 211771 | LLP778_Traptavidin-p65HSF1-CO | AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection |
| 211772 | LLP779_αGCN4-VP64-CO | SunTag counterpart binding domain, αGCN4, fused to transcriptional activator VP64, with GFP selection |
| 211773 | LLP780_SpyCatcher-KRAB-CO | SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection |
| 211774 | LLP781_SnoopCatcher-KRAB-CO | SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection |
| 211775 | LLP782_Traptavidin-KRAB-CO | AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection |
| 211776 | LLP783_SpyCatcher-DNMT3A-CO | SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection |
| 211777 | LLP784_SnoopCatcher-DNMT3A-CO | SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection |
| 211778 | LLP785_Traptavidin-DNMT3A-CO | AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection |
| 211779 | LLP786_SpyCatcher-EZH2-FL-CO | SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection |
| 211780 | LLP787_SnoopCatcher-EZH2-FL-CO | SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection |
| 211781 | LLP788_Traptavidin-EZH2-FL-CO | AviTag counterpart binding domain, Traptavidin, fused to polycomb repressive complex subunit EZH2, with GFP selection |
| 211782 | LLP618_αGCN4-KRAB-CO | SunTag counterpart binding domain, aGCN4, fused to transcriptional repressor KRAB, with GFP selection |
| 211783 | LLP789_αGCN4-DNMT3A-CO | SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection |
| 211784 | LLP790_αGCN4-EZH2-FL-CO | SunTag counterpart binding domain, aGCN4, fused to polycomb repressive complex subunit EZH2, with GFP selection |
| 211785 | LLP617_αGCN4-mCherry | SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection |
| 211786 | LLP791_PB_dCas9-SSSavi_BirA | PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection |