RHINO directs MMEJ to repair DNA breaks in mitosis.

Brambati A, Sacco O, Porcella S, Heyza J, Kareh M, Schmidt JC, Sfeir A
Science. 2023 Aug 11;381(6658):653-660. doi: 10.1126/science.adh3694. Epub 2023 Jul 13. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
211535	hLig4 guide1 in pX458	sgRNA-1 against Lig4
211536	hLig4 guide2 in pX458	sgRNA-2 against Lig4
211537	pLenti-GFP-2A-Puro-gATRIP_A	sgRNA-A against ATRIP
211538	pLenti-GFP-2A-Puro-gATRIP_B	sgRNA-B against ATRIP
211539	pLenti-GFP-2A-Puro-gETAA1_A	sgRNA-A against ETAA1
211540	pLenti-GFP-2A-Puro-gETAA1_B	sgRNA-B against ETAA1
211541	pLenti-GFP-2A-Puro-gHUS1_A	sgRNA-A against HUS1
211542	pLenti-GFP-2A-Puro-gHUS1_B	sgRNA-B against HUS1
211543	pLenti-GFP-2A-Puro-gIL25_A	sgRNA-A against IL25
211544	pLenti-GFP-2A-Puro-gRAD1_A	sgRNA-A against RAD1
211545	pLenti-GFP-2A-Puro-gRAD1_B	sgRNA-B against RAD1
211611	pLenti-GFP-2A-Puro-gRAD9A_A	sgRNA-A against RAD9
211612	pLenti-GFP-2A-Puro-gRAD9A_B	sgRNA-B against RAD9
211613	pLenti-GFP-2A-Puro-gRHNO1_A	sgRNA-A against Rhno
211614	pLenti-GFP-2A-Puro-gRHNO1_B	sgRNA-B against Rhno
211616	pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro	Rhno1 Plk1mut7A
211617	pLenti-h.Rhno1 Cdk1mut7A-C-Myc-P2A-Puro	Rhno1 Cdk1mut7A
211619	pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-Puro	Rhno1 delta TOPBP1 (T202A)
211620	pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro	Rhno1 S51A
211621	pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro	Rhno1 T52A
211622	pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro	Rhno1 S141A
211623	pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro	Rhno1 S162A
211624	pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro	Rhno1 S163A
211625	pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro	Rhno1 S164A
211626	pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro	Rhno1 S178A
211627	PX458 pSp Cas9 Rhino1 tagA	Cas9 Rhino1 tagA
211628	lenti-CHMP4B gRNA1	sgRNA-1 against CHMP4B
211629	lenti-CHMP4B gRNA2	sgRNA-2 against CHMP4B
211630	lenti-ERCC1 gRNA1	sgRNA-1 against ERCC1
211631	lenti-ERCC1 gRNA2	sgRNA-2 against ERCC1
211632	lenti-ERCC6L gRNA1	sgRNA-1 against ERCC6L
211633	lenti-ERCC6L gRNA2	sgRNA-2 agsinst ERCC6L
211634	lenti-ZWILCH gRNA1	sgRNA-1 against ZWILCH
211635	lenti-ZWILCH gRNA2	sgRNA-2 against ZWILCH
211636	gRNA-POLQ-Halo	sgRNA against POLQ
211637	HDR-HaloPOLQ	halo donor