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Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition.

Nam M, Xia W, Mir AH, Jerrett A, Spinelli JB, Huang TT, Possemato R
Nat Metab. 2024 Dec;6(12):2338-2353. doi: 10.1038/s42255-024-01166-w. Epub 2024 Nov 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
211519pLentiCRISPR-v2 sgCASP8Deletes CASP8
211520pLentiCRISPR-v2 sgBAK1Deletes BAK1
211521pLentiCRISPR-v2 sgUGP2Deletes UGP2
211522pLentiCRISPR-v2 sgUCK2Deletes UCK2
211523pLentiCRISPR-v2 sgUCKL1Deletes UCKL1
211524pLentiCRISPR-v2 hygro sgUCK1Deletes UCK1
211525pSECB sgCASP9Deletes CASP9
211526BPK1520 sgRPS3Facilitate knock-in of RPS3-Keima fusion protein
211527BPK1520 sgFADDDeletes FADD
211528LV-sgRNA-mCherry-Puro sgPIDDDeletes PIDD
211529pLJM1 flag-cFLIPExpresses flag-tagged cFLIP (CFLAR)
211530pMXS-IRES-Blast coUCK2 D62AExpresses codon optimized catalytic mutant UCK2
211531pMXS-IRES-Blast coUGP2Expresses codon optimized UGP2
211533pCW57.1 blast tBID-flagExpresses truncated BID upon doxycycline addition to trigger apoptosis
211534pLentiCRISPR-v2 sgATG7Deletes ATG7

Antibodies from Article