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CRISPR-Cas9 for selective targeting of somatic mutations in pancreatic cancers.

Teh SSK, Bowland K, Bennett A, Halper-Stromberg E, Skaist A, Tang J, Cai F, Macoretta A, Liang H, Kamiyama H, Wheelan S, Lin MT, Hruban RH, Scharpf RB, Roberts NJ, Eshleman JR
bioRxiv. 2023 Oct 10:2023.04.15.537042. doi: 10.1101/2023.04.15.537042. Preprint. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200941lentiGuide-puro_Panc480-MT7Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone
211471pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidinlentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance
211822pLenti_mNeonGreen-blast-GACTLentiviral vector for fluorescence and barcoding of human cells

Antibodies from Article