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CRISPR-Cas9 for selective targeting of somatic mutations in pancreatic cancers.

Teh SSK, Bowland K, Halper-Stromberg E, Kotwal A, Bennett A, Skaist A, Tang J, Cai F, Macoretta A, Liang H, Kamiyama H, Wheelan S, Lin MT, Hruban RH, Hung CF, Goldstein M, Scharpf RB, Roberts NJ, Eshleman JR
NAR Cancer. 2024 Jun 19;6(2):zcae028. doi: 10.1093/narcan/zcae028. eCollection 2024 Jun. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
193780pLentiCas9-T2A-mAppleTricistronic expression of Cas9-mApple-BSD in mammalian cells
193781pLenti-dCas9-T2A-GFPTricistronic expression of dCas9-EGFP-BSD
200941lentiGuide-puro_Panc480-MT7Multiplexed CRISPR array expressing 7 sgRNA in a lentiviral backbone
211471pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidinlentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

Antibodies from Article