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Prominin 1 and Tweety Homology 1 both induce extracellular vesicle formation.

Bell TA, Luce BE, Hakim P, Ananda VY, Dardari H, Nguyen TH, Monshizadeh A, Chao LH
Elife. 2024 Aug 13;13:e100061. doi: 10.7554/eLife.100061. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
210816pCS2-HsProm1s1-TwinStrep-HisMammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)
210817pCS2-HsProm1s1(W795R)-TwinStrep-HisMammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)
210818pCDNA3.1-HsPcdh21-Myc-Flag-HisMammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)
210819pCDNA3.1-HsPcdh21-mNeonGreenMammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)
210820pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-FlagMammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)
210821pCS2-HsProm1s1-StayGoldMammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)
210822pCS2-HsProm1s1(W795R)-StayGoldMammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)
210823pET28a-mScarlet-TEV-Strep-HisBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein
210824pET28a-mTagBFP2-TEV-Strep-HisBacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein
211330pCS2-HsProm1s1-mScarletMammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)
211332pLX304-HsTtyh1-TwinStrep-HisMammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)
211333AAVS-HsProm1s1-StayGoldVector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)
211334AAVS-HsProm1s1(W795R)-StayGoldVector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

Antibodies from Article