Developing small Cas9 hybrids using molecular modeling
Mangin A, Dion V, Menzies G
bioRxiv 2023.10.24.563270
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210735 | pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2 | Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats |
| 210736 | pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2 | Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats |
| 210737 | pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2 | Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1 |
| 210738 | pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2 | Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2 |
| 210739 | pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2 | Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3 |
| 210740 | pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2 | Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4 |
| 210741 | pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1 |
| 210742 | pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2 |
| 210743 | pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3 |
| 210744 | pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4 |
| 210745 | pUC57-ITR2-H1-GFP sgRNA Gollum Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 1 |
| 210746 | pUC57-ITR2-H1-GFP sgRNA Gollum Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 2 |
| 210747 | pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3 |
| 210748 | pUC57-ITR2-H1-GFP sgRNA Gollum Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2 | Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 4 |
| 210749 | pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2 | Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats |
| 222857 | pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2 | This plasmid codes for the Slug-KH nickase with a sg(CTG)6 |
| 222858 | pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2 | This plasmid codes for the Slug-KH nickase with a sg(CAG)6 |
| 222859 | pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2 | This plasmid codes for the Slug-KH nickase with a sg(AGC)6 |
| 222860 | pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2 | This plasmid codes for the Slug-KH nickase with a sg(GCA)6 |
| 222861 | pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA | This plasmid codes for the OgeuIscB nickase |
| 222862 | OgeuIscB GCT RNA | This plasmid codes for the guide of the OgeuIscB with a GCT target |
| 222863 | OgeuIscB TGC RNA | This plasmid codes for the guide of the OgeuIscB with a TGC target |
| 222864 | OgeuIscB GCA RNA | This plasmid codes for the guide of the OgeuIscB with a GCA target |
| 222865 | OgeuIscB CTG RNA | This plasmid codes for the guide of the OgeuIscB with a CTG target |
| 222866 | OgeuIscB AGC RNA | This plasmid codes for the guide of the OgeuIscB with a AGC target |
| 222867 | OgeuIscB CAG RNA | This plasmid codes for the guide of the OgeuIscB with a CAG target |