Directed stepwise tracing of polysynaptic neuronal circuits with replication-deficient pseudorabies virus.

Du W, Li E, Guo J, Arano R, Kim Y, Chen YT, Thompson A, Oh SJ, Samuel A, Li Y, Oyibo HK, Xu W
Cell Rep Methods. 2023 Jun 16;3(6):100506. doi: 10.1016/j.crmeth.2023.100506. eCollection 2023 Jun 26. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
201992	pAAV-TRE-IEo	AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus
210729	AAV-Dlx-SynaptoTAG	AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.