Synonymous codon usage regulates translation initiation.
Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, Stasevich TJ, Rissland OS
Cell Rep. 2023 Dec 26;42(12):113413. doi: 10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210568 | pUB_SMFLuc0---MS2 | Expresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpins |
| 210570 | pUB_SMFLuc100---MS2 | Expresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpins |
| 210571 | pIS1_Opt_Renilla | Expresses Optimal Renilla luciferase |
| 210572 | pUB_SM-RLuc0---MS2 | Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins |
| 210573 | pUB_SM-RLuc100---MS2 | Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins |
| 210574 | pUB_FLuc100---MS2 | Expresses Optimal Firefly with 3'UTR MS2 hairpins |
| 210575 | pcDNA3.1_λHA-GFPopt | Expresses lambdaHA-optimal GFP |
| 210576 | pcDNA3.1_λHA-GFPnonopt | Expresses lambdaHA-nonoptimal GFP |
| 210577 | pUB_OptλHAramp-FLuc100 | Expresses Optimal lambda HA-Optimal Firefly luciferase |
| 210579 | pUB_NonoptλHAramp-FLuc100 | Expresses Nonoptimal lambda HA-Optimal Firefly luciferase |
| 210580 | pcDNA3.1_SM-FLuc0-malat1 | Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix |
| 210581 | pcDNA3.1_SM-FLuc100-malat1 | Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix |
| 210583 | pcDNA3.1_SM-FLuc0-pA | Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA |
| 210584 | pcDNA3.1_SM-FLuc100-pA | Expresses SM(FLAG)-optimal Firefly luciferase - pA |
| 210589 | Dm_λHA-FLuc0_CSC | Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila) |
| 210590 | pUB_SM-FLuc0_EMCV-Ren | Expresses SM(FLAG)-nonoptimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase |
| 210591 | pUB_SM-FLuc100_EMCV-Ren | Expresses SM(FLAG)-optimal Firefly luciferase, and EMCVIRES-driven Renilla luciferase |
| 210592 | Dm_λHA-FLuc100_CSC | Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila) |
| 210593 | Dm_Opt_Renilla_CSC | Expresses Optimal Renilla luciferase (CSC metric; Drosophila) |
| 210594 | pUB_SM-FLuc0-GFPOPT | Expresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFP |
| 210596 | pUB_SM-Fluc100-GFPOPT | Expresses SM(FLAG)-optimal Firefly luciferase-optimal GFP |