Using CombiCells, a platform for titration and combinatorial display of cell surface ligands, to study T-cell antigen sensitivity modulation by accessory receptors.
Patel A, Andre V, Eguiguren SB, Barton MI, Burton J, Denham EM, Pettmann J, Morch AM, Kutuzov MA, Siller-Farfan JA, Dustin ML, van der Merwe PA, Dushek O
EMBO J. 2024 Jan;43(1):132-150. doi: 10.1038/s44318-023-00012-1. Epub 2023 Dec 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210565 | pHR-SIN-BX SpyCatcher003-hCD52 | Expresses Spycatcher003 on the cell surface using human CD52 hinge |
| 210567 | pHR-SIN-BX SpyCatcher003-(short)mCD80 | Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain |
| 210664 | pTT3-ecdCD86-ST3-His | Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar) |
| 210665 | pTT3-ecdCD80-ST3-His | Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar) |
| 210666 | pTT3-ecdICAM1-ST3-His | Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar) |
| 223219 | pTT3-ecdCD19-ST3-His_withSUMO | Expression of the extracellular domain of human CD19 fused to Spytag003 + Histag in HEK293(or similar) with SUMO for better protein expression |
| 223220 | pET-HLA-A0201-ST3 | Expression of human HLA A2 heavy chain fused to Spytag003 for expression in bacteria |
| 223221 | pET14-H2Kb-ST3 | Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria |