Massively parallel in vivo Perturb-seq reveals cell-type-specific transcriptional networks in cortical development.
Zheng X, Wu B, Liu Y, Simmons SK, Kim K, Clarke GS, Ashiq A, Park J, Li J, Wang Z, Tong L, Wang Q, Rajamani KT, Munoz-Castaneda R, Mu S, Qi T, Zhang Y, Ngiam ZC, Ohte N, Hanashima C, Wu Z, Xu X, Levin JZ, Jin X
Cell. 2024 Jun 20;187(13):3236-3248.e21. doi: 10.1016/j.cell.2024.04.050. Epub 2024 May 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210523 | pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR | AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology. |
| 210525 | pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR | AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology. |
| 213480 | pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR | AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology. |
| 213481 | pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR | AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology. |
| 224435 | pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR | AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology. |