Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs following a whole-genome duplication event.
Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, Gagnon-Arsenault I, Landry CR
Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210472 | pKB33 (pUC19-KANMX-tetO7-CYCpr) | Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration. |
| 210481 | pDN0520 (pDEST, TRP1) | pDEST vector with TRP1 marker for gene expression in S. cerevisiae. |
| 210482 | pDN0603 (pDEST, NatR) | pDEST vector with NatR marker for gene expression in S. cerevisiae. |
| 210483 | pDN0604 (pDEST, HygR) | pDEST vector with HygR marker for gene expression in S. cerevisiae. |
| 210484 | pDN0617 (pDEST-mEGFP C-term, NatR) | pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae. |
| 210485 | pKB11 (pDEST-DHFR F[1,2] C-term, NatR) | pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae. |
| 210486 | pKB12 (pDEST-DHFR F[3] C-term, HygR) | pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae. |
| 210487 | pDN0605 (pDEST-DHFR F[1,2] N-term, NatR) | pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae. |
| 210488 | pDN0606 (pDEST-DHFR F[3] N-term,HygR) | pDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae. |