Recombinant Expression, Purification, and Assembly of p62 Filaments.
Tarafder AK, Guesdon A, Kuhm T, Sachse C
Methods Mol Biol. 2019;1880:3-15. doi: 10.1007/978-1-4939-8873-0_1.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
210425 | pETM43 p62 FL (1-440) | Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site |
210426 | pETM43 p62 dUBA (1-389) | Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site |
210427 | pOPTM p62 PB1 (1-102) | Bacterial expression of MBP-PB1(1-102) with TEV cleavage site |
210428 | pOPTM p62 PB1 (1-122) | Bacterial expression of MBP-PB1(1-122) with TEV cleavage site |
210429 | pOPTM p62 PB1 ZZ (1-167) | Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site |