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Recombinant Expression, Purification, and Assembly of p62 Filaments.

Tarafder AK, Guesdon A, Kuhm T, Sachse C
Methods Mol Biol. 2019;1880:3-15. doi: 10.1007/978-1-4939-8873-0_1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
210425pETM43 p62 FL (1-440)Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site
210426pETM43 p62 dUBA (1-389)Bacterial expression of MBP-p62 dUBA (1-389) with 3C cleavage site
210427pOPTM p62 PB1 (1-102)Bacterial expression of MBP-PB1(1-102) with TEV cleavage site
210428pOPTM p62 PB1 (1-122)Bacterial expression of MBP-PB1(1-122) with TEV cleavage site
210429pOPTM p62 PB1 ZZ (1-167)Bacterial expression of MBP-PB1 ZZ (1-167) with TEV cleavage site

Antibodies from Article