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From Llama to Nanobody: A Streamlined Workflow for the Generation of Functionalised VHHs.

Eyssen LE, Ramadurai S, Abdelkarim S, Buckle I, Cornish K, Lin H, Jones AK, Stephens GJ, Owens RJ
Bio Protoc. 2024 Mar 20;14(6):e4962. doi: 10.21769/BioProtoc.4962. eCollection 2024 Mar 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
210402pOPINVHH_BAP_hisFor expression of VHH with C-terminal biotin acceptor peptide and his tag
210403pOPINVHH_cys_hisFor expression of VHH with C-terminal cysteine and his tag
210404pOPINVHH_his_flagFor expression of VHH with C-terminal his and Flag tags
210405pOPINVHH_hisFor expression of VHH with C-terminal his tag
210406pOPINVHH_sortase_hisFor expression of VHH with C-terminal sortase and his tags
210407pOPINVHH_snap_hisFor expression of VHH with C-terminal snap protein and his tag
210526pOPINVHH_his_mycFor expression of VHH with C-terminal his and myc tags

Antibodies from Article