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From Llama to Nanobody: A Streamlined Workflow for the Generation of Functionalised VHHs.

Eyssen LEA, Ramadurai S, Abdelkarim S, Buckle I, Cornish K, Lin H, Jones AK, Stephens GJ, Owens RJ.
Bio-protocol 14(6): e4962. (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
210402pOPINVHH_BAP_hisFor expression of VHH with C-terminal biotin acceptor peptide and his tag
210403pOPINVHH_cys_hisFor expression of VHH with C-terminal cysteine and his tag
210404pOPINVHH_his_flagFor expression of VHH with C-terminal his and Flag tags
210405pOPINVHH_hisFor expression of VHH with C-terminal his tag
210406pOPINVHH_sortase_hisFor expression of VHH with C-terminal sortase and his tags
210407pOPINVHH_snap_hisFor expression of VHH with C-terminal snap protein and his tag
210526pOPINVHH_his_mycFor expression of VHH with C-terminal his and myc tags

Antibodies from Article