The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus replication.
Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi M, Ueberheide B, Stapleford KA, Pagano M
J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210312 | NSP3C-FR-2Strep | Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells |
| 210315 | NSP3-FR-2Strep | Expresses SARS-CoV-2 NSP3 in mammalian cells |
| 210316 | NSP4-FR-2Strep | Expresses SARS-CoV-2 NSP4 in mammalian cells |
| 210317 | NSP6-FR-2Strep | Expresses SARS-CoV-2 NSP6 in mammalian cells |
| 210318 | NSP3C-EGFP | Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells |
| 210319 | NSP4-mCherry | Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells |
| 210339 | NSP4-WT-Flag | Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells |
| 210340 | NSP4-H120N/F121L-Flag | Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells |
| 210341 | NSP4-Δ220-234-Flag | Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells |
| 210342 | PcDNA3.1-Flag | Empty Vector for molecular cloning |
| 210343 | PcDNA3.1-2Strep | Empty Vector for molecular cloning |
| 210344 | NSP2-Flag | Expresses SARS-CoV-2 NSP2 in mammalian cells |
| 210345 | NSP6-Flag | Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells |
| 210346 | NSP14-Flag | Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells |
| 210347 | Flag-V0A | Expresses ATP6V0A in mammalian cells |
| 210348 | Flag-V0D1 | Expresses ATP6V0D1 in mammalian cells |
| 210349 | NSP3C-Flag | Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells |
| 210350 | REEP5-2Strep | Expresses REEP5 fused with 2Strep in mammalian cells |
| 210351 | REEP6-2Strep | Expresses REEP6 fused with 2Strep in mammalian cells |
| 210352 | TRAM1-2Strep | Expresses TRAM1 fused with 2Strep in mammalian cells |
| 210353 | SGTA-2Strep | Expresses SGTA fused with 2Strep in mammalian cells |
| 210354 | DNAJC11-2Strep | Expresses DNAJC11 fused with 2Strep in mammalian cells |
| 210355 | IDE-2Strep | Expresses IDE fused with 2Strep in mammalian cells |
| 210356 | XPO6-2Strep | Expresses XPO6 fused with 2Strep in mammalian cells |
| 210357 | FKBP10-2Strep | Expresses FKBP10 fused with 2Strep in mammalian cells |
| 210359 | ATG9A-2STrep | Expresses ATG9A fused with 2Strep in mammalian cells |
| 210360 | TMEM106B-2Strep | Expresses TMEM106B fused with 2Strep in mammalian cells |
| 210361 | STIM1-2Strep | Expresses STIM1 fused with 2Strep in mammalian cells |
| 210362 | GLG1-2Strep | Expresses GLG1 fused with 2Strep in mammalian cells |
| 210363 | REEP5-EGFP | Expresses REEP5 fused with EGFP in mammalian cells |
| 210364 | REEP6-EGFP | Expresses REEP6 fused with EGFP in mammalian cells |
| 210365 | TRAM1-EGFP | Expresses TRAM1 fused with EGFP in mammalian cells |
| 210366 | TMEM106B-EGFP | Expresses TMEM106B fused with EGFP in mammalian cells |
| 210367 | ATG9A-EGFP | Expresses ATG9A fused with EGFP in mammalian cells |
| 210368 | DNAJC11-EGFP | Expresses DNAJC11 fused with EGFP in mammalian cells |
| 210369 | FKBP10-EGFP | Expresses FKBP10 fused with EGFP in mammalian cells |
| 210370 | STIM1-EGFP | Expresses STIM1 fused with EGFP in mammalian cells |
| 210371 | XPO6-EGFP | Expresses XPO6 fused with EGFP in mammalian cells |
| 210372 | GLG1-EGFP | Expresses GLG1 fused with EGFP in mammalian cells |
| 210373 | SGTA-EGFP | Expresses SGTA fused with EGFP in mammalian cells |
| 210374 | TRAM2-2Strep | Expresses TRAM2 fused with 2Strep in mammalian cells |
| 210375 | SEC61B-2Strep | Expresses SEC61B fused with 2Strep in mammalian cells |
| 210376 | SARS-CoV-2 N-2Strep | Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells |
| 210377 | NSP3C-mCherry | Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells |
| 210378 | NSP4-EGFP | Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells |