Protocol for Allele-Specific Epigenome Editing Using CRISPR/dCas9.
Rajaram N, Bashtrykov P, Jeltsch A
Methods Mol Biol. 2024;2842:179-192. doi: 10.1007/978-1-0716-4051-7_9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210212 | sgRNA_expression_vector | An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI. |