Protocol for allele specific epigenome editing using CRISPR/dCas9

Rajaram N, Bashtrykov P, Jeltsch A
Methods Mol Biol, in press.

Plasmids from Article

ID	Plasmid	Purpose
210212	sgRNA_expression_vector	An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.