High-throughput PRIME-editing screens identify functional DNA variants in the human genome.
Ren X, Yang H, Nierenberg JL, Sun Y, Chen J, Beaman C, Pham T, Nobuhara M, Takagi MA, Narayan V, Li Y, Ziv E, Shen Y
Mol Cell. 2023 Dec 21;83(24):4633-4645.e9. doi: 10.1016/j.molcel.2023.11.021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210188 | LentiV2-EF1α-nCas9/RT | Expression of nCas9/RT-P2A-Puro |
| 210189 | LentiV2-hU6-evopreQ1 | pegRNA/ngRNA library cloning |
| 210190 | LentiV2-mU6-evopreQ1 | pegRNA/ngRNA library cloning |
| 214697 | sgRNA scaffold-spacer-human U6 | provide "sgRNA scaffold-spacer-human U6" fragment |
| 214698 | sgRNA scaffold-spacer-mouse U6 | provide "sgRNA scaffold-spacer-mouse U6" fragment |