Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR libraries.
Nuttle X, Burt ND, Currall B, Moyses-Oliveira M, Mohajeri K, Bhavsar R, Lucente D, Yadav R, Tai DJC, Gusella JF, Talkowski ME
Cell Rep Methods. 2023 Dec 5:100672. doi: 10.1016/j.crmeth.2023.100672.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210028 | pPB9v1 | Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro |
| 210029 | pPB9v2 | Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro |
| 210030 | pPB9P2 | Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro |
| 210032 | pPB1163 | Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast |
| 210033 | pPB758 | Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro |
| 210034 | pPB9P3 | Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro |
| 210035 | pPB12A | Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro |
| 210036 | pPB9 | Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP |