Development of a Golden Gate Assembly-Based Genetic Toolbox for Lactiplantibacillus plantarum and Its Application for Engineering Monoterpenoid Biosynthesis.
Li X, Schönberg PY, Wucherpfennig T, Hinze C, Sulaj F, Henle T, Mascher T
ACS Synth Biol. 2024 Sep 20;13(9):2764-2779. doi: 10.1021/acssynbio.4c00075. Epub 2024 Sep 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209958 | pLP3 | Entry vector to store Level 0 broad-host range L. plantarum replication origin parts |
| 209959 | pLP4 | Entry vector to store Level 0* parts |
| 209960 | pLP5 | Entry vector to store Level 0* resistance cassette parts |
| 209961 | pLP6 | Entry vector to store Level 0* replication origin parts |
| 209962 | pLPC53 | Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids |
| 209963 | pLPP1 | Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids |
| 209964 | pLPP2 | Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids |
| 209965 | pLPP3 | Contains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmids |
| 209966 | pLPP4 | Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids |
| 209967 | pLPP5 | Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids |
| 209968 | pLPP6 | Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion |
| 209969 | pLPP7 | Contains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmids |
| 209970 | pLPP8 | Contains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmids |
| 209971 | pLPP9 | Contains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmids |
| 209972 | pLPS2 | Contains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmids |
| 209973 | pLP-EV | Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids |
| 209974 | pLPT2 | Contains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmids |
| 209975 | pLPO1 | Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids |
| 209976 | pLPO2 | Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids |
| 209977 | pLPO3 | Contains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmids |
| 209978 | pLPO4 | Contains Level 0 Part: replication origin (pWV01) for the construction of Level 1 plasmids |
| 209979 | pLPR1 | Contains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmids |
| 209980 | pLPR2 | Contains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmids |
| 209981 | pLPE1 | Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids |
| 209982 | pLPE2 | Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids |
| 209983 | pLPC54 | Contains Level 0* Part: 5' Connector (5C_07_5C1C) for the construction of Level 2 plasmids |
| 209984 | pLPC33 | Contains Level 0* Part: 3' Connector (3C_08_3C3) for the construction of Level 2 plasmids |
| 209985 | pLPE3 | Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids |
| 209986 | pLPR3 | Contains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmids |
| 209987 | pLPO5 | Contains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmids |