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Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for Visualizing Membrane Contact Site Dynamics.

Miner GE, Smith SY, Showalter WK, So CM, Ragusa JV, Powers AE, Zanellati MC, Hsu CH, Marchan MF, Cohen S
Contact (Thousand Oaks). 2024 Feb 4;7:25152564241228911. doi: 10.1177/25152564241228911. eCollection 2024 Jan-Dec. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
209858Cyto-BDimerization dependent fluorescent protein B fused to NES
209859Cyto-GADimerization dependent fluorescent protein GA fused to NES
209860Cyto-RADimerization dependent fluorescent protein RA fused to NES
209861LD-BDimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
209862LD-GADimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
209863LD-RADimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
209864Mito-BDimerization dependent fluorescent protein B anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
209865Mito-GADimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
209866Mito-RADimerization dependent fluorescent protein R anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVS
209867Perox-BDimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
209868Perox-GADimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
209869Perox-RADimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
209870ER-BDimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
209871ER-GADimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
209872ER-RADimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
209873Lyso-BDimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1
209874Lyso-GADimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1
209875Lyso-RADimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1
209876PM-BDimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43
209877PM-GADimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43
209878PM-RADimerization dependent fluorescent protein RA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43
209879Cav-BDimerization dependent fluorescent protein B anchored to cytosolic face of the caveolae with mouse CAVIN1
209880Cav-GADimerization dependent fluorescent protein GA anchored to cytosolic face of the caveolae with mouse CAVIN1
209881Cav-RADimerization dependent fluorescent protein RA anchored to cytosolic face of the caveolae with mouse CAVIN1

Antibodies from Article