Direct observation of motor protein stepping in living cells using MINFLUX.
Deguchi T, Iwanski MK, Schentarra EM, Heidebrecht C, Schmidt L, Heck J, Weihs T, Schnorrenberg S, Hoess P, Liu S, Chevyreva V, Noh KM, Kapitein LC, Ries J
Science. 2023 Mar 10;379(6636):1010-1015. doi: 10.1126/science.ade2676. Epub 2023 Mar 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196975 | pET28a_DmKHC[1-421]-SNAP-6xHis | Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag |
| 213403 | pHTN_HaloTag_KIF5B[1-560] | Truncated human kinesin-1 with HaloTag at the N-terminus |
| 213404 | pHTN_HaloTag_KIF5B | Full-length human kinesin-1 with HaloTag at the N-terminus |