MicroRNA-122-Mediated Liver Detargeting Enhances the Tissue Specificity of Cardiac Genome Editing.
Yang L, Liu Z, Chen G, Chen Z, Guo C, Ji X, Cui Q, Sun Y, Hu X, Zheng Y, Li Y, Gao F, Chen L, Zhou P, Pu WT, Guo Y
Circulation. 2024 May 28;149(22):1778-1781. doi: 10.1161/CIRCULATIONAHA.123.065438. Epub 2024 May 28.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209781 | AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS | Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter |
| 209782 | AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS | Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter |
| 209783 | AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS | Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR) |
| 209784 | pAAV-cTNT-Cre-miR122 TS | Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR) |
| 209785 | AAV-cTNT-Cre | Expresses Cre by the specific cTNT promoter |
| 209786 | pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN | Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter |
| 209787 | pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS | Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR) |
| 209788 | pAAV-CASI-InteinC-SpRY C aa714-1368-U6-Camk2d sgRNA | Expresses SpRY cas9C by the constitutive CASI promoter and sgRNA targeting the oxidative activation sites of Camk2d by U6 promoter |