Optogenetic Microwell Array Screening System: A High-Throughput Engineering Platform for Genetically Encoded Fluorescent Indicators.
Rappleye M, Wait SJ, Lee JD, Siebart JC, Torp L, Smith N, Muster J, Matreyek KA, Fowler DM, Berndt A
ACS Sens. 2023 Nov 24;8(11):4233-4244. doi: 10.1021/acssensors.3c01573. Epub 2023 Nov 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209760 | pAAV_hsyn_HA-Flag-uMASS_1_WPRE | AAV virus production for neuronal expression of uMASS_1 under hSyn promoter |
| 209761 | pAAV_hsyn_HA_Flag-uMASS_LF | AAV virus production for neuonal expression of uMASS (loss-of-function) under hSyn promoter |
| 209762 | pCAG-uMASS_1 | Expression of uMASS_1 in mammalian cells under CAG promoter |
| 209763 | pCAG--uMASS_LF | Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter |
| 209764 | pCMV-dMASS_1 | Expression of dMASS_1 in mammalian cells under CMV promoter |
| 209765 | pAAV-hsyn-dMASS_1_WPRE | AAV virus production for neuronal expression of dMASS_1 under hSyn promoter |
| 209766 | pAAV-hsyn-dMASS_LF_WPRE | AAV virus production for neuronal expression of dMASS_LF (loss-of-function) under hSyn promoter |
| 213393 | pAAV_EF1a_DIO_HA/FLAG_muMASS | uMASS_1 opioid sensor. AAV Virus production for Cre dependent expression |
| 213394 | pAAV_EF1a_DIO_HA/FLAG_muMASS_LF | Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression. |