Low-affinity ligands of the epidermal growth factor receptor are long-range signal transmitters in collective cell migration of epithelial cells.

Deguchi E, Lin S, Hirayama D, Matsuda K, Tanave A, Sumiyama K, Tsukiji S, Otani T, Furuse M, Sorkin A, Matsuda M, Terai K
Cell Rep. 2024 Nov 14;43(11):114986. doi: 10.1016/j.celrep.2024.114986. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
209893	pCSIIpuro-EGF-ScNeo	To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209894	pCSIIpuro-HBEGF-ScNeo	To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209895	pCSIIpuro-TGFa-ScNeo	To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209896	pCSIIpuro-EREG-ScNeo	To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209897	pCSIIpuro-AREG-ScNeo	To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209898	pCSIIpuro-BTC-ScNeo	To monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209899	pCSIIpuro-EPGN-ScNeo	To monitor the status of Epigen, the plasmid encodes a recombinant Epigen fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209900	pCSIIpuro-NRG1-ScNeo	To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209901	pCSIIpuro-Necl5-ScNeo	To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209902	pPBbleo-EGF-ScNeo	To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209903	pPBbleo-HBEGF-ScNeo	To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209904	pPBbleo-TGFa-ScNeo	To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209905	pPBbleo-EREG-ScNeo	To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209906	pPBpuro-NRG1-ScNeo	To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209907	pPBbsr2-HBEGF-ScNeo	To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209908	pPBbsr2-TGFa-ScNeo	To monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209909	pPBbsr2-EREG-ScNeo	To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209910	pPBbsr2-AREG-ScNeo	To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209911	pPBbsr2-NRG1-ScNeo	To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
209912	pCSIIpuro-HBEGF-mNeonGreen	To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein
209913	pCSIIpuro-TGFa-mNeonGreen	To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein
209914	pCSIIpuro-EREG-mNeonGreen	To monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent protein
209915	pPBbleo-TGFa-EREG-ScNeo	To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen
209916	pPBbsr2-TSen	A FRET biosensor for ADAM17 activity
209917	pPBbsr2-5102HRasCT(Picchu)	A FRET biosensor for tyrosine kinase activity
209918	pCSIIbsr-Eevee-ROCK-NES	A FRET biosensor for ROCK activity
209919	pPBpuro-miRFP703-eDHFR(69K6)-cRaf	Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)
209920	pPBbleo-miRFP703-eDHFR(69K6)-cRaf	Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)
209921	pPBbsr2-miRFP703-eDHFR(69K6)-cRaf	Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)
209922	LentiCRISPRv2puro-E-cadherin(Canis)-exon1 6-28 gRNA	A knock-out vector for the dog CDH1
209923	LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA	A knock-out vector for the dog CTNND1