iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP.
Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown TA, Ryan TA
Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 10.1073/pnas.2314604121. Epub 2024 May 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209651 | pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag | Expression of iATPSnFR2, high affinity |
| 209652 | pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag | Expression of iATPSnFR2, medium affinity |
| 209653 | pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag | Expression of iATPSnFR2, low affinity |
| 209654 | pAAV.CAG.(cyto).cpSFGFP.HaloTag | Non-responsive control |
| 209655 | pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag | Expression of iATPSnFR2, high affinity |
| 209656 | pAAV.CAGFLEX.(cyto).iATPSnFR2.A95K.HaloTag | Expression of iATPSnFR2, medium affinity |
| 209657 | pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag | Expression of iATPSnFR2, low affinity |
| 209658 | pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag | Non-responsive control |
| 209659 | pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag | Expression of iATPSnFR2, high affinity |
| 209660 | pAAV.GFAP.(cyto).iATPSnFR2.A95K.HaloTag | Expression of iATPSnFR2, medium affinity |
| 209661 | pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag | Expression of iATPSnFR2, low affinity |
| 209662 | pAAV.GFAP.(cyto).cpSFGFP.HaloTag | Non-responsive control |
| 209663 | pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag | Expression of iATPSnFR2, high affinity |
| 209664 | pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag | Expression of iATPSnFR2, medium affinity |
| 209665 | pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag | Expression of iATPSnFR2, low affinity |
| 209666 | pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag | Non-responsive control |
| 209722 | pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag | Expresses mitochondrially targeted low affinity ratiometric ATP sensor |
| 209723 | pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3 | Expresses mitochondrially targeted low affinity ratiometric ATP sensor |
| 209724 | plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag | Expresses cytosolic low affinity ratiometric ATP sensor |
| 209725 | plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3 | Expresses cytosolic low affinity ratiometric ATP sensor |
| 209726 | plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag | Expresses cytosolic medium affinity ratiometric ATP sensor |
| 209727 | plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3 | Expresses cytosolic medium affinity ratiometric ATP sensor |
| 209728 | plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3 | Expresses cytosolic high affinity ratiometric ATP sensor |
| 209729 | plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag | Expresses presynaptic low affinity ratiometric ATP sensor |
| 209730 | plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3 | Expresses presynaptic low affinity ratiometric ATP sensor |
| 214925 | pAAV.CAG.(mito).cpSFGFP.HaloTag | Expresses mitochondrially targeted non-responsive control |
| 214926 | plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3 | Expresses presynaptic non-responsive control |