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An Optimised CRISPR Cas9 and Cas12a Toolkit for Barley and Wheat

Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Harwood W
Research Square, 12 March 2024, PREPRINT (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
209449EC70188 Cloning protospacers for Cas9 editing
209450EC70189Cloning protospacers for Cas9 editing
209451EC70190Cloning protospacers for Cas9 editing
209452EC70191 Cloning protospacers for Cas9 editing
209453EC70192Cloning protospacers for Cas9 editing
209454EC70193 Cloning protospacers for Cas9 editing
209455EC70194Cloning protospacers for Cas9 editing
209456EC70195Cloning protospacers for Cas9 editing
209457EC70196Cloning protospacers for Cas9 editing
209458EC70197Cloning protospacers for Cas9 editing
209459EC70198Cloning protospacers for Cas9 editing
209460EC70199Cloning protospacers for Cas9 editing
209461EC70200Cloning protospacers for Cas9 editing
209462EC70201Cloning protospacers for Cas9 editing
209463EC70202Cloning protospacers for Cas9 editing
209464EC70203Cloning protospacers for Cas9 editing
209465EC70204Cloning protospacers for Cas9 editing
209466EC70205Cloning protospacers for Cas9 editing
209467EC70206Cloning protospacers for Cas9 editing
209468EC70207Cloning protospacers for Cas9 editing
209469EC70208Cloning protospacers for Cas9 editing
209470EC67904Cloning protospacers for Cas12a editing
209471EC67845Cloning protospacers for Cas12a editing
209472EC67846Cloning protospacers for Cas12a editing
209473EC67847Cloning protospacers for Cas12a editing
209474EC67848Cloning protospacers for Cas12a editing
209475EC67849Cloning protospacers for Cas12a editing
209476EC67850Cloning protospacers for Cas12a editing
209477EC70209Cloning protospacers for Cas12a editing
209478EC70210Cloning protospacers for Cas12a editing
209479EC70121Cloning protospacers for Cas12a editing
209480EC70122Cloning protospacers for Cas12a editing
209481EC70123Cloning protospacers for Cas12a editing
209482EC70124Cloning protospacers for Cas12a editing
209483EC70211Cloning protospacers for Cas12a editing
209484EC70212Cloning protospacers for Cas12a editing
209485EC70213Cloning protospacers for Cas12a editing
209486EC70125Cloning protospacers for Cas12a editing
209487EC70126Cloning protospacers for Cas12a editing
209488EC70127Cloning protospacers for Cas12a editing
209489EC70128Cloning protospacers for Cas12a editing
209490EC70214Cloning protospacers for Cas12a editing
209491EC67897Cloning protospacers for Cas12a editing
209492EC67898Cloning protospacers for Cas12a editing
209493EC67899Cloning protospacers for Cas12a editing
209494EC67900Cloning protospacers for Cas12a editing
209495EC67901Cloning protospacers for Cas12a editing
209496EC67902Cloning protospacers for Cas12a editing
209497EC67903Cloning protospacers for Cas12a editing
209498EC67891Cloning protospacers for Cas12a editing
209499EC67892Cloning protospacers for Cas12a editing
209500EC70129Cloning protospacers for Cas12a editing
209501EC70130Cloning protospacers for Cas12a editing
209502EC70131Cloning protospacers for Cas12a editing
209503EC70132Cloning protospacers for Cas12a editing
209504EC67893Cloning protospacers for Cas12a editing
209505EC67894Cloning protospacers for Cas12a editing
209506EC67895Cloning protospacers for Cas12a editing
209507EC70133Cloning protospacers for Cas12a editing
209508EC70134Cloning protospacers for Cas12a editing
209509EC70135Cloning protospacers for Cas12a editing
209510EC70136Cloning protospacers for Cas12a editing
209511EC67896Cloning protospacers for Cas12a editing
209512EC67841Cloning in complete guide expression cassettes, with Cas12a (D156R)
209513EC67843Cloning in complete guide expression cassettes, with Cas12a (D156R)
209514EC67844Cloning in complete guide expression cassettes, with Cas9
209515EC70364Cloning in complete guide expression cassettes, with Cas9
209516EC67842Cloning in complete guide expression cassettes, with Cas12a (D156R)
209517EC64420Cloning in complete guide expression cassettes, with Cas9
209518EC64434Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)
211795EC67908Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

Antibodies from Article