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The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana.

Tan H, Zhou Y, Dinius E, Lozano-Duran R
J Integr Plant Biol. 2024 Jan 31. doi: 10.1111/jipb.13610. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
209378pGW-GOI-TurboIDGateway compatible plasmid for TurboID-based proximity labelling experiment in planta
209379pGW-GOI-TurboID-GFPGateway compatible plasmid for TurboID-based proximity labelling experiment in planta
209380pGW-TurboID-GOIGateway compatible plasmid for TurboID-based proximity labelling experiment in planta
209381pGW-GFP-TurboID-GOIGateway compatible plasmid for TurboID-based proximity labelling experiment in planta
209382pGW-GFP-GOI-TurboIDGateway compatible plasmid for TurboID-based proximity labelling experiment in planta
209383pGW-TurboID-GOI-GFPGateway compatible plasmid for TurboID-based proximity labelling experiment in planta
209385LI B-C TurboIDGolden Gate LI B-C module
209386LI D-E TurboIDGolden Gate LI D-E module
209387LI D-E TurboID-GFPGolden Gate LI D-E module
209388pGG-GOI-DE-TurboIDGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta
209389pGG-GOI-DE-TurboID-GFPGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta
209390pGG-BC-TurboID-GOIGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta
209391pGG-TurboIDTransiently expressing TurboID in planta
209392pGG-TurboID-3XNLSTransiently expressing TurboID fused with 3X nuclear localization signal (NLS) in planta
209393pGG-NES-TurboIDTransiently expressing TurboID fused with a nuclear export signal (NES) in planta
209394pGG-cTP-TurboIDTransiently expressing TurboID fused with a chloroplast transit peptide (cTP) in planta
209395pGG-PM-TurboIDTransiently expressing TurboID fused with a plasma menbrane targeting peptide in planta
209396pGG-mTP-TurboIDTransiently expressing TurboID fused with a Mitochondrial transit peptide (mTP) in planta
209397pGG-GTS-TurboIDTransiently expressing TurboID fused with a Golgi targeting sequence (GTS) in planta
209398pGG-TurboID-PTS1Transiently expressing TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta
209399pGG-TurboID-GFPTransiently expressing and visualizing the subcellular localization of TurboID in planta
209400pGG-GFP-TurboID-3XNLSTransiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in planta
209401pGG-NES-TurboID-GFPTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in planta
209402pGG-GFP-PM-TurboIDTransiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta
209403pGG-cTP-TurboID-GFPTransiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta
209404pGG-mTP-TurboID-GFPTransiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in planta
209405pGG-GTS-TurboID-GFPTransiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta
209406pGG-TurboID-GFP-PTS1Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

Antibodies from Article