High-throughput protein characterization by complementation using DNA barcoded fragment libraries.

Biggs BW, Price MN, Lai D, Escobedo J, Fortanel Y, Huang YY, Kim K, Trotter VV, Kuehl JV, Lui LM, Chakraborty R, Deutschbauer AM, Arkin AP
Mol Syst Biol. 2024 Oct 7. doi: 10.1038/s44320-024-00068-z. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
209325	pBWB507	p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.
209326	pBWB514	p15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.