Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects.

Gopalakrishna KP, Hillebrand GH, Bhavana VH, Elder JL, D'Mello A, Tettelin H, Hooven TA
Commun Biol. 2023 Jun 9;6(1):620. doi: 10.1038/s42003-023-04994-w. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
209317	p3015b	Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.