Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription.
Wilkinson ME, Frangieh CJ, Macrae RK, Zhang F
Science. 2023 Apr 21;380(6642):301-308. doi: 10.1126/science.adg7883. Epub 2023 Apr 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209290 | pMW41: pET-His-MBP-SUMO-R2Bm-SII | Expresses the R2Bm ORF in E. coli |
| 209291 | pMW37: pRS426-R2Bm RNA | Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences |
| 209292 | pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII | Expresses R2Bm-Cas9(H840A) fusion in E. coli |