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Untargeted proteomics identifies plant substrates of the bacterial-derived ADP-ribosyltransferase AvrRpm1

Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, Susanne Matschi, eIvan Matić, and Lennart Wirthmueller
bioRxiv 2023.09.25.558804 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
209185pENTR4 AvrRpm1pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tags
209186pENTR4 CDS A. thaliana MAMIpENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tags
209187pENTR4 CDS A. thaliana PHOT1pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags
209188pENTR4 genomic A. thaliana PLDGAMMA3pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags
228395pENTR4 HopU1pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags
228396pOPINF AtMKK5Recombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coli
228397pOPINF HopF2Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli

Antibodies from Article