The juxtamembrane linker of synaptotagmin 1 regulates Ca(2+) binding via liquid-liquid phase separation.
Mehta N, Mondal S, Watson ET, Cui Q, Chapman ER
Nat Commun. 2024 Jan 4;15(1):262. doi: 10.1038/s41467-023-44414-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209005 | pETSUMOSyt1C2AB(80-421) | Recombinant protein expression of truncated syt1 construct |
| 209006 | pETSUMOSyt1C2AB(96-421) | Recombinant protein expression of truncated syt1 construct |
| 209007 | pETSUMOSyt1C2AB(143-421) | Recombinant protein expression of truncated syt1 construct |
| 209008 | pETSUMOSyt1C2AB(80 JuxtaK-421) | Recombinant protein expression of truncated syt1 construct with Juxta K mutations |
| 209009 | pETSyt1C2AB(80-421)msGFP | Recombinant protein expression of truncated syt1 tagged with GFP |
| 209010 | pETSyt1C2AB(143-421)msGFP | Recombinant protein expression of truncated syt1 tagged with GFP |
| 209011 | pETSyt1C2AB(80 JuxtaK-421)msGFP | Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP |
| 209012 | pETmsGFPSyt1(80-142) | Recombinant protein expression of truncated syt1 tagged with GFP |
| 209013 | pETmsGFPSyt1(80 JuxtaK-142) | Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP |
| 209014 | pFSynWSyt1C2AB(80-421)msGFP | Overexpression of truncated syt1 tagged with GFP in mammalian cells |
| 209015 | pFSynWSyt1C2AB(143-421)msGFP | Overexpression of truncated syt1 tagged with GFP in mammalian cells |
| 209016 | pFSynWSyt1C2AB(80 JuxtaK-421)msGFP | Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells |