General method for rapid purification of native chromatin fragments.

Kuznetsov VI, Haws SA, Fox CA, Denu JM
J Biol Chem. 2018 Aug 3;293(31):12271-12282. doi: 10.1074/jbc.RA118.002984. Epub 2018 May 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
213504	pT22-GluCNZ-6His	Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.