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Essential gene knockdowns reveal genetic vulnerabilities and antibiotic sensitivities in Acinetobacter baumannii.

Ward RD, Tran JS, Banta AB, Bacon EE, Rose WE, Peters JM
mBio. 2023 Dec 21:e0205123. doi: 10.1128/mbio.02051-23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
208888pAbaMCi-GFP-BsaI_pJMP2748Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).
208889pAbaMCi-GFP-gfp_pJMP2768Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).
208890pAbaMCi-BsaI_pJMP2776Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

Antibodies from Article