Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD
Adriaenssens E, Nguyen TN, Sawa-Makarska J, Khuu G, Schuschnig M, Shoebridge S, Watts EM, Csalyi KD, Padman BS, Lazarou M, Martens S
bioRxiv September 25, 202
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208862 | pHAGE-FKBP-GFP-NAP1 | Stably express GFP-tagged NAP1 for CID induced mitophagy |
| 208863 | pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52) | Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy |
| 208864 | pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200) | Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy |
| 208865 | pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1) | Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy |
| 208866 | pHAGE-FKBP-GFP-OPTN | Stably express GFP-tagged OPTN for CID induced mitophagy |
| 208867 | pHAGE-FKBP-GFP-OPTN (2-119) | Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy |
| 208868 | pBMN-HA-NAP1 | For mammalian expression of NAP1 |
| 208869 | pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1) | For mammalian expression of NAP1 (delta-TBK1) |
| 208870 | pGEX-4T1-GST-TEV-GFP-NAP1 | For expression in E. coli of GFP-NAP1 |
| 208871 | pGEX-4T1-MBP-TEV-NAP1 (WT) | For expression in E. coli of NAP1 |
| 208872 | pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E) | For expression in E. coli of NAP1 (delta-NDP52) |
| 208873 | pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q) | For expression in E. coli of NAP1 (delta-TBK1) |
| 208874 | pFastBac-Dual-GST-TEV-SINTBAD-mCherry | For expression in insect cells of SINTBAD-mCherry |
| 208875 | pFastBac-Dual- GST-TEV-TBK1 | For expression in insect cells of TBK1 |
| 210209 | pBMN-HA-SINTBAD | For mammalian expression of SINTBAD (Internal ref. SMC2249) |
| 216836 | pGEX4T1-GST-LC3B | For expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463) |
| 216837 | pcDNA3.1_NAP1-eGFP | For mammalian expression of NAP1-GFP (Internal ref. SMC1638) |
| 216840 | pHAGE-FKBP-GFP-SINTBAD | Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy |
| 216843 | pET-Duet_OPTN-thrombin-GST | For expression in E. coli of OPTN (Internal ref. SMC1789) |
| 217610 | pGEX-4T1-GST-TEV-NAP1 | For expression in E. coli of NAP1 |