TLNRD1 is a CCM complex component and regulates endothelial barrier integrity

Neil J. Ball, Sujan Ghimire, Gautier Follain, Ada O. Pajari, Monika Vaitkevičiūtė, Alana R. Cowell, Bence Berki, Johanna Ivaska, ProfileIlkka Paatero, Benjamin T. Goult, Guillaume Jacquemet
BioRxiv Article

Plasmids from Article

ID	Plasmid	Purpose
208861	TLNRD1_5H_eGFP	Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.
208876	CCM2_wt_eGFP	Express eGFP-CCM2 in mammalian cells.
208877	CCM2_d410-444_eGFP	Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.
208878	CCM2_I428S_eGFP	Express eGFP-CCM2 with the I428S mutation in mammalian cells.
208879	CCM2_I432D_eGFP	Express eGFP-CCM2 with the I432D mutation in mammalian cells.
208880	CCM2_W421AD422A_eGFP	Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.
208881	TLNRD1_35-345_eGFP	Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.
208883	TLNRD1_2T_eGFP	Express eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.
208884	TLNRD1_2E_eGFP	Express eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.
208886	mito-CCM2-mScarlet-I	Express mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells.
208887	mito-PDCD10-mScarlet-I	Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.