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Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters.

Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, Kirkland TA, Brennan CK, Prescher JA, Amelio AL
iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. eCollection 2024 Sep 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
208838pLenti_CMV:Flag-CeNLucExpresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.
208839pLenti_CMV:Flag-CeNLExpresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.
208840pLenti_CMV:Flag-GpNLucExpresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.
208841pLenti_CMV:Flag-GeNLExpresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.
208849pLenti_CMV:Flag-YeNLExpresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.
208850pLenti_CMV:Flag-OeNLExpresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.
208851pLenti_CMV:Flag-OgNLucExpresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.
208852pLenti_CMV:Flag-AntaresExpresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.
208854pLenti_CMV:Flag-LumiscarletExpresses FLAG epitope-tagged Lumiscarlet "Red" BRET reporter in mammalian cells.
208855pLenti_CMV:Flag-KaNLucExpresses FLAG epitope-tagged KaNLuc "Red" BRET reporter in mammalian cells.
208856pLenti_CMV:Flag-dKeNLucExpresses FLAG epitope-tagged dKeNLuc "Red" BRET reporter in mammalian cells.

Antibodies from Article