The structural mechanism of dimeric DONSON in replicative helicase activation.
Cvetkovic MA, Passaretti P, Butryn A, Reynolds-Winczura A, Kingsley G, Skagia A, Fernandez-Cuesta C, Poovathumkadavil D, George R, Chauhan AS, Jhujh SS, Stewart GS, Gambus A, Costa A
Mol Cell. 2023 Nov 16;83(22):4017-4031.e9. doi: 10.1016/j.molcel.2023.09.029. Epub 2023 Oct 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208796 | pRSFDuet-1-Cdc45 | Xenopus laevis FLAG5-His10-TEV-Cdc45 |
| 208797 | pET28a-DONSON | Xenopus laevis His6-Myc-DONSON wild type |
| 208798 | pET28a-DONSON-WM | Xenopus laevis His6-Myc-DONSON (W234L, M463T) |
| 208799 | pET28a-DONSON-DEWS | Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A) |
| 208800 | pET28a-DONSON-3A | Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A) |
| 208801 | pET28a-DONSON-Δ24 | Xenopus laevis His6-Myc-DONSON (1-24aa removed) |
| 208802 | pET28a-DONSON-DEW | Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A) |
| 208803 | pET28a-DONSON-S437A | Xenopus laevis His6-Myc-DONSON (S437A) |
| 208804 | pET28a-DONSON-ΔD1 | Xenopus laevis His6-DONSON (1-155aa removed) |